Profile LDH BFY B is a recombinant human Lactate Dehydrogenase B protein produced in E. coli, supplied as a purified, sterile-filtered solution. It catalyzes the reversible interconversion of pyruvate and lactate while coupling NAD+ and NADH, making it essential for cellular energy production and a biomarker for certain pathological conditions. The product is available either as an active enzyme reagent or as part of an immunocapture assay kit designed to measure LDH-B activity in diagnostic and research applications.
Technical Specifications
• Source: Recombinant human protein expressed in E. coli
• Amino Acid Composition: 354 amino acids (residues 1–334) with N-terminal His-Tag (20 a.a.)
• Molecular Weight: 38.8 kDa polypeptide; approximately 40.8 kDa including His-Tag
• Purity: >95.0% by SDS-PAGE
• Specific Activity: >6 units/mg (one unit = 1.0 µmole pyruvate converted to L-lactate and β-NAD per minute at pH 7.5, 37°C)
• Formulation: Sterile-filtered, colorless solution in 20 mM Tris-HCl pH 8, 1 mM DTT, 10% glycerol
• Dye Extinction Coefficient (assay kit): 37,000 M⁻¹ cm⁻¹ (yellow product, absorbance at 450 nm)
– Key Features
• His-Tag enables rapid purification and detection
• >95% purity ensures minimal contamination in downstream applications
• Specific activity >6 units/mg provides reliable quantitation
• Immunocapture assay principle yields colorimetric readout at 450 nm
• Species cross-reactivity confirmed for human, goat, and bovine samples; no reactivity with mouse or rat sera
– Storage & Stability
• Short-term storage: 4°C for 2–4 weeks
• Long-term storage: −20°C; add 0.1% HSA or BSA as carrier protein
• Minimize freeze-thaw cycles to preserve biological activity
– Typical Applications
• Enzyme activity assays and metabolic studies
• Diagnostic marker detection for germ cell tumors and cardiac tissue injury
• Recombinant protein research and validation
• Assay calibration and quality control
– Compatibility & Integration
LDH-B substrate specificity permits integration into NAD+/NADH-coupled assay platforms. The His-Tag facilitates immobilization on affinity supports. Colorimetric output (450 nm) is compatible with standard plate readers and spectrophotometric instrumentation.
