The MPD LAB3055 is a diagnostic assay for quantifying 25-hydroxyvitamin D (total D2/D3) in serum, delivering the most accurate measurement of vitamin D nutritional status. This test evaluates both ergocalciferol (D2) and cholecalciferol (D3) metabolites, which play essential roles in bone and mineral metabolism. The assay processes serum specimens collected in SST (gold) tubes or lithium heparin alternatives, requiring minimal sample volumes and straightforward specimen handling protocols.
Technical Specifications
• Analyte: 25-Hydroxycalciferol (25-OH-D)
• CPT Code: 82306
• Specimen Type: Serum (preferred), Lithium Heparin plasma (alternate)
• Collection Containers: SST (gold) tube for serum; lithium heparin (light green) tube for alternate
• Minimum Volume: 0.3 mL serum
• Preferred Volume: 1.0 mL serum
• Clotting Time: Up to 60 minutes
• Centrifugation Time: 15 minutes
• Processing Requirement (non-gel tubes): Transfer plasma to labeled tube within 2 hours of collection
– Key Features
• Measures both D2 and D3 forms in a single assay
• Gold-standard marker for vitamin D status assessment
• Accommodates minimal sample volumes without repeat-testing capability at minimum volume
• Compatible with standard serum collection workflows
– Typical Applications
Vitamin D nutritional assessment in clinical laboratory environments. The test distinguishes between dietary sources (D2 from vegetables, yeast, fungi) and endogenous synthesis (D3 from cutaneous sun exposure and oily fish), both converted to 25-hydroxyvitamin D in hepatic metabolism.
– Compatibility & Integration
Accepts serum from standard SST collection tubes and lithium heparin plasma. Aligns with routine centrifugation protocols and standard laboratory processing timelines.
